Blastocyst Culture in IVF – Why it Should be Done?

Rahul Khorwal By Rahul Khorwal, 26th Jun 2017 | Follow this author | RSS Feed | Short URL http://nut.bz/1y1d9i_x/
Posted in Wikinut>Health>General Health>Women's Health

An embryo can be transferred fresh into the uterus this day, be cultured an additional day to day-6 if awaiting a genetic test result for fresh transfer, or be cryopreserved by vitrification and stored for a later month awaiting other genetic test results.

Embryo cycle using IVF

Cutting edge laboratory technology is allowing embryos to grow in our incubators for an extended period of time–up to six days. By six days of laboratory growth, the embryo must either be transferred into the woman’s uterus or cryopreserved and stored in liquid nitrogen for transfer at some future time. Reproduction across all species on the planet is not very efficient. Not all eggs, sperm or embryos they form are normal. Errors of nature commonly occur in the formation and growth of embryos. If cars and cell phones were of equal efficiency as normal human reproduction, product performance failure would force the manufacturers out of business for the sake of customer dissatisfaction alone! One of the common errors in embryo development is related to genetics. Not necessarily abnormal genetics of the parents, but rather abnormal genetics of the egg, sperm or the process of evenly cutting the genetic deck of 46 chromosomes within any one cell of an embryo with every cell division while growing. Errors in chromosome number within embryo cells account for about two thirds of embryos failing to implant or failing to sustain life beyond the first 8 weeks of pregnancy, resulting in miscarriage.

Two distinct sets of cells separate:

The majority of cells press outward against the interior of the embryo shell called the trophectoderm and will become the placenta. About 12-14 cells huddle together within the fluid space while still connected to the trophectoderm. These cells, called the inner cell mass, will become the fetus and baby at birth. If preimplantation genetic testing of the embryo is planned, you can imagine the fluid pressure building from within the embryo may cause some of the trophectoderm cells to push out through the laser induced “coin slot” created on day-3. These cells will extend outside the confines of the embryo shell like a hernia. About 5 of these cells are excised with a laser and undergo genetic testing. This is called a trophectoderm biopsy. These cells have a high reliability to represent the same genome as the inner cell mass that will form the fetus. Thus, the genetic test results of cells destined to form the placenta should be similar to the genetics of the cells that will form the baby. Only obtaining cells that push to the outside of the embryo shell, protects the embryo as a whole, still within the shell, from harm during this biopsy procedure.

Embryo cycle using IVF

An embryo can be transferred fresh into the uterus this day, be cultured an additional day to day-6 if awaiting a genetic test result for fresh transfer, or be cryopreserved by vitrification and stored for a later month awaiting other genetic test results.

Advantages of Extended Embryo Culture and Blastocyst Transfer

• Precision in selection of a high quality embryo.
• Embryos that advance to blastocysts stage are more likely to be genetically competent.
• Opportunity to more reliably assess genetic competency through preimplantation genetic testing.
• Reduction in the probability of miscarriage.

Avoiding selection and transfer of a genetically abnormal embryo with normal microscopic appearance on day 3, but is truly “fixed to fail” leading to failure to become pregnant or to remain pregnant- resulting in miscarriage. For women with advanced reproductive age and women with diminished ovarian reserve, time is of the essences. Extended embryo culture and transferring a blastocyst with normal preimplantation genetic testing results (if at least one embryo forms normally) reduces the possibility of miscarriage and the typical 4-month delay from known pregnancy to recovery from miscarriage needed before trying to become pregnant again.

Tags

Blastocyst Culture, Ivf, Ivf Technique

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author avatar Rahul Khorwal
I'm a Digital Marketing Executive. I believe to Internet will feature of world.

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